IJMS, Vol. 18, Pages 1765: Correlation of Leukocyte Telomere Length Measurement Methods in Patients with Dyskeratosis Congenita and in Their Unaffected Relatives

IJMS, Vol. 18, Pages 1765: Correlation of Leukocyte Telomere Length Measurement Methods in Patients with Dyskeratosis Congenita and in Their Unaffected Relatives

<p>International Journal of Molecular Sciences <a href="http://ift.tt/2w1WBZ9">doi: 10.3390/ijms18081765</a></p> 
<p>Authors: 
    Payal Khincha 
    Casey Dagnall 
    Belynda Hicks 
    Kristine Jones 
    Abraham Aviv 
    Masayuki Kimura 
    Hormuzd Katki 
    Geraldine Aubert 
    Neelam Giri 
    Blanche Alter 
    Sharon Savage 
    Shahinaz Gadalla 
    </p> 
<p>Several methods have been employed to measure telomere length (TL) in human studies. It has been difficult to directly compare the results from these studies because of differences in the laboratory techniques and output parameters. We compared TL measurements (TLMs) by the three most commonly used methods, quantitative polymerase chain reaction (qPCR), flow cytometry with fluorescence in situ hybridization (flow FISH) and Southern blot, in a cohort of patients with the telomere biology disorder dyskeratosis congenita (DC) and in their unaffected relatives (controls). We observed a strong correlation between the Southern blot average TL and the flow FISH total lymphocyte TL in both the DC patients and their unaffected relatives (R2 of 0.68 and 0.73, respectively). The correlation between the qPCR average TL and that of the Southern blot method was modest (R2 of 0.54 in DC patients and of 0.43 in unaffected relatives). Similar results were noted when comparing the qPCR average TL and the flow FISH total lymphocyte TL (R2 of 0.49 in DC patients and of 0.42 in unaffected relatives). In conclusion, the strengths of the correlations between the three widely used TL assays (qPCR, flow FISH, and Southern blot) were significantly different. Careful consideration is warranted when selecting the method of TL measurement for research and for clinical studies.</p><br /><br />

from # & – All via ola Kala on Inoreader http://ift.tt/2w1WBZ9

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